Given below are two statements Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of r…

Question

Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below:

1. A. Both statement I and statement II are correct (Correct answer)
2. B. Both statement I and statement II are incorrect
3. C. Statement I is correct but statement II is incorrect
4. D. Statement I is incorrect but statement II is correct

Correct Answer

Option A — Both statement I and statement II are correct

Detailed Solution & Explanation

The correct answer is Both statement I and statement II are correct.

Key Points

• DNA bands separated on an agarose gel are cut out and extracted (elution); these purified fragments are joined with cloning vectors to construct recombinant DNA — Statement I is correct.
• In gel electrophoresis DNA moves from the cathode (wells) toward the anode; smaller fragments travel farther (near the anode) while larger ones stay near the wells — Statement II is correct.