Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes, B. DNA ligase, C. DNA mutase, D. DNA…

Question

Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes, B. DNA ligase, C. DNA mutase, D. DNA recombinase, E. DNA polymerase.

Choose the correct answer from the options given below:

1. A. C and D only (Correct answer)
2. B. A and B only
3. C. D and E only
4. D. B and C only

Correct Answer

Option A — C and D only

Detailed Solution & Explanation

The correct answer is C and D only.

Key Points

• Gene cloning primarily uses restriction enzymes (cutting), DNA ligase (joining) and DNA polymerase (replication) — A, B and E are essential.
• DNA mutase and DNA recombinase are not used in the standard gene-cloning process, so C and D are the answer.